In a world where Humans and Cartoons live side by side, Part time Private investigator and full time drunk Eddie Valiant is hired to investigate the wife of Cartoon Star Roger Rabbit. After showing the poor toon some photos of his aforementioned wife, Jessica, playing “pattycake” with Famed Producer Marvin Acme, Roger goes ballistic and disappears into the dark alleys of tinsel town. The following morning, Eddie learns that Marvin Acme has been Murdered and Roger is the primary suspect, but when the Rabbit on the Lab turns up at Valiant and Valiant Detective agency looking for help, Eddie finds himself in the middle of a conspiracy to wipe toon town off the face of the earth.